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One of the priority research area of our laboratory is proteinological and proteomic analysis of various Parkinson’s disease model systems and biological materials obtained from patients with Parkinson’s disease, Miyoshi syndrome/myopathy, or MIDD (maternaly inherited diabetes and deafness) – MELAS (Mitochondrial Encephalopathy, Lactic Acidosis, Stroke-like episodes) spectrum syndrome.
Due to the fact that these are disorders associated with systemic, metabolic and/or nerve degeneration, which is directly related to damaged mitochondrial homeostasis and cell energy profile, another priority area of research in our laboratory is focused on the etiopathogenesis of primary and secondary mitochondriopathies.
Watch a vide tour of our Laboratory of Proteomics and Mitochondriopathies.
The core technologies used in our laboratory are common techniques of molecular, biological and genetic analyses, which are combine with state-of-the-art MALDI-TOF mass spectrometry, ESI-IT mass spectrometry and NMR spectroscopy. These technologies allow us to analyze both the proteome and the phospho-proteome of cells, which combines very well with the subsequent analysis aimed at evaluating the activity of the signalome using a chip technology.
Measurements of oxygen consumption at the cellular level (basal respiration, maximum respiration capacity, respiratory reserve) and bioenergetic profile are usually determined in cell cultures or in white blood cells isolated from venous blood using an Oroboros O2k respirometer. Circulating cells in patients are specific in a way that come into contact with all organs in the body, and thus usually react to various pathological conditions and cause changes. The outputs of this method can serve as biomarker (s) of complex cellular energy.
Mitochondrial dysfunction (so called mitochondrial respiratory disorders) is associated with various degenerative diseases (such as mitochondrial encephalopathy, Parkinson’s and Alzheimer’s disease, Kearns-Sayre syndrome, MIDD / MELAS syndrome, diabetes, muscular dystrophies, ALS, schizophrenia). The study of this dysfunction in relation to the above-mentioned diseases is of great importance, both from a scientific or clinical point of view. Determination of bioenergetic profile is also justified in the field of preventive medicine, for example, in diagnostics, therapy and research of metabolic syndrome and obesity, or cardiovascular diseases.
Take a virtual tour of our modern Laboratory of Proteomics and Mitochondriopathies.
Kolísek M. (principal investigator), source: Government of the Slovak Republic – project “Back home”, title: Influence of deletion of Na + / Mg2 + exchanger SLC41A1 and mitochondrial Na + -dependent Mg2 + exporter SLC41A3 on brain morphology and molecular composition in mouse MPTP-induced Parkinsonian model; solution period: 2016-2017
Kolísek M. (co-investigator), Tatarková Z. (principal investigator), source: Agency for Science and Research, number: APVV-SK-KR-18-0001, title: Mitochondrial-like nanoparticles in the treatment of Parkinson’s disease and other degenerative diseases; solution period: 2018-2019
Kolísek M. (principal investigator), source: Scientific Grant Agency of the Ministry of Education, Youth and Sports of the Slovak Republic and SAS, number: VEGA 1/0554/19, title: The role of the STAT3 signalling pathway in the regulation of the Na + / Mg2 + promoter of the SLC41A1 exchanger: from inflammation to Parkinson’s disease; solution time: 2019-2022
Kolísek M. (principal investigator), source: Agency for Science and Research, number: APVV-19-0222, title: Determination of mitochondrial fitness in the diagnostics and prediction of Parkinson’s disease; solution period: 2020-2024
Kolísek M. (co-investigator), Hnilicová Petra (principal investigator) source: Agency for Science and Research, number: APVV-SK-AT-20-0010, title: Multiparametric examinations of Miyoshi myopathy using magnetic resonance modalities; solution time: 2021-2022
Kolísek M. (principal investigator), source: Government of the Slovak Republic – project “Back home”, title: Influence of deletion of Na + / Mg2 + exchanger SLC41A1 and mitochondrial Na + -dependent Mg2 + exporter SLC41A3 on brain morphology and molecular composition in mouse MPTP-induced Parkinsonian model; solution period: 2016-2017
Kolísek M. (co-investigator), Tatarková Z. (principal investigator), source: Agency for Science and Research, number: APVV-SK-KR-18-0001, title: Mitochondrial-like nanoparticles in the treatment of Parkinson’s disease and other degenerative diseases; solution period: 2018-2019
Kolísek M. (principal investigator), source: Scientific Grant Agency of the Ministry of Education, Youth and Sports of the Slovak Republic and SAS, number: VEGA 1/0554/19, title: The role of the STAT3 signalling pathway in the regulation of the Na + / Mg2 + promoter of the SLC41A1 exchanger: from inflammation to Parkinson’s disease; solution time: 2019-2022
Kolísek M. (principal investigator), source: Agency for Science and Research, number: APVV-19-0222, title: Determination of mitochondrial fitness in the diagnostics and prediction of Parkinson’s disease; solution period: 2020-2024
Kolísek M. (co-investigator), Hnilicová Petra (principal investigator) source: Agency for Science and Research, number: APVV-SK-AT-20-0010, title: Multiparametric examinations of Miyoshi myopathy using magnetic resonance modalities; solution time: 2021-2022
The most important publications
Jurecek L, Rajcigelova T, Kozarova A, Werner T, Vormann J, Kolisek M. J Beneficial effects of an alkaline topical treatment in patients with mild atopic dermatitis. Cosmet Dermatol. 2021 Jan 14. doi: 10.1111/jocd.13936.
Brodnanova M, Hatokova Z, Evinova A, Cibulka M, Racay P. Differential impact of imipramine on thapsigargin- and tunicamycin-induced endoplasmic reticulum stress and mitochondrial dysfunction in neuroblastoma SH-SY5Y cells. Eur J Pharmacol. 2021 Mar 30:174073. doi: 10.1016/j.ejphar.2021.174073.
Pokusa M, Hajdúchová D, Menichová V, Evinová A, Hatoková Z, Kráľová-Trančíková A. Vulnerability of subcellular structures to pathogenesis induced by rotenone in SH-SY5Y cells. Physiol Res. 2021 Mar 17;70(1):89-99. doi: 10.33549/physiolres.934477.
Evinova A, Cizmarova B, Hatokova Z, Racay P. J Correction to: High-Resolution Respirometry in Assessment of Mitochondrial Function in Neuroblastoma SH-SY5Y Intact Cells. Membr Biol. 2020 Apr;253(2):137. doi: 10.1007/s00232-020-00112-7. PMID: 32146487
Evinova A, Cizmarova B, Hatokova Z, Racay P. High-Resolution Respirometry in Assessment of Mitochondrial Function in Neuroblastoma SH-SY5Y Intact Cells. J Membr Biol. 2020 Apr;253(2):129-136. doi: 10.1007/s00232-020-00107-4.
Jurek S, Sandhu MA, Trappe S, Bermúdez-Peña MC, Kolisek M, Sponder G, Aschenbach JR. Optimizing adipogenic transdifferentiation of bovine mesenchymal stem cells: a prominent role of ascorbic acid in FABP4 induction. Adipocyte. 2020 Dec;9(1):35-50. doi: 10.1080/21623945.2020.1720480.
Tatarkova Z, de Baaij JHF, Grendar M, Aschenbach JR, Racay P, Bos C, Sponder G, Hoenderop JGJ, Röntgen M, Turcanova Koprusakova M, Kolisek M. Dietary Mg2+ Intake and the Na+/Mg2+ Exchanger SLC41A1 Influence Components of Mitochondrial Energetics in Murine Cardiomyocytes. Int J Mol Sci. 2020 Nov 3;21(21):8221. doi: 10.3390/ijms21218221.
Hnilicová P, Štrbák O, Kolisek M, Kurča E, Zeleňák K, Sivák Š, Kantorová E. Current Methods of Magnetic Resonance for Noninvasive Assessment of Molecular Aspects of Pathoetiology in Multiple Sclerosis. Int J Mol Sci. 2020 Aug 25;21(17):6117. doi: 10.3390/ijms21176117.
Cibulka M, Brodnanova M, Grendar M, Grofik M, Kurca E, Pilchova I, Osina O, Tatarkova Z, Dobrota D, Kolisek M.SNPs rs11240569, rs708727, and rs823156 in SLC41A1 Do Not Discriminate Between Slovak Patients with Idiopathic Parkinson’s Disease and Healthy Controls: Statistics and Machine-Learning Evidence. Int J Mol Sci. 2019 Sep 21;20(19):4688. doi: 10.3390/ijms20194688.
Werner T, Kolisek M, Vormann J, Pilchova I, Grendar M, Struharnanska E, Cibulka M. Assessment of bioavailability of Mg from Mg citrate and Mg oxide by measuring urinary excretion in Mg-saturated subjects. Magnes Res. 2019 Aug 1;32(3):63-71. doi: 10.1684/mrh.2019.0457.
Chiang YF, Chen HY, Lee IT, Chien LS, Huang JH, Kolisek M, Cheng FC, Tsai SW. Magnesium-responsive genes are downregulated in diabetic patients after a three-month exercise program on a bicycle ergometer. J Chin Med Assoc. 2019 Jun;82(6):495-499. doi: 10.1097/JCMA.0000000000000112.
Kolisek M, Sponder G, Pilchova I, Cibulka M, Tatarkova Z, Werner T, Racay P. Magnesium Extravaganza: A Critical Compendium of Current Research into Cellular Mg2+ Transporters Other than TRPM6/7. Rev Physiol Biochem Pharmacol. 2019;176:65-105. doi: 10.1007/112_2018_15.
Merolle L, Sponder G, Sargenti A, Mastrototaro L, Cappadone C, Farruggia G, Procopio A, Malucelli E, Parisse P, Gianoncelli A, Aschenbach JR, Kolisek M, Iotti S. Metallomics. Overexpression of the mitochondrial Mg channel MRS2 increases total cellular Mg concentration and influences sensitivity to apoptosis. 2018 Jul 18;10(7):917-928. doi: 10.1039/c8mt00050f.
Sponder G, Abdulhanan N, Fröhlich N, Mastrototaro L, Aschenbach JR, Röntgen M, Pilchova I, Cibulka M, Racay P, Kolisek M. Overexpression of Na+/Mg2+ exchanger SLC41A1 attenuates pro-survival signaling. Oncotarget. 2017 Dec 22;9(4):5084-5104. doi: 10.18632/oncotarget.23598. eCollection 2018 Jan 12.
Miersch C, Stange K, Hering S, Kolisek M, Viergutz T, Röntgen M. Molecular and functional heterogeneity of early postnatal porcine satellite cell populations is associated with bioenergetic profile. Sci Rep. 2017 Mar 27;7:45052. doi: 10.1038/srep45052.
Kolisek M, Touyz RM, Romani A, Barbagallo M. Magnesium and Other Biometals in Oxidative Medicine and Redox Biology. Oxid Med Cell Longev. 2017;2017:7428796. doi: 10.1155/2017/7428796. Epub 2017 Oct 4. PMID: 29109833
Sandhu MA, Jurek S, Trappe S, Kolisek M, Sponder G, Aschenbach JR. Influence of Bovine Serum Lipids and Fetal Bovine Serum on the Expression of Cell Surface Markers in Cultured Bovine Preadipocytes. Cells Tissues Organs. 2017;204(1):13-24. doi: 10.1159/000472708.
Mastrototaro L, Smorodchenko A, Aschenbach JR, Kolisek M, Sponder G. Solute carrier 41A3 encodes for a mitochondrial Mg(2+) efflux system. Sci Rep. 2016 Jun 15;6:27999. doi: 10.1038/srep27999.
The result of the EU-funded BioMed Martin project is a revolutionary research center that develops new diagnostic and therapeutic methods and at the same time strengthens cooperation between the city of Martin and the Žilina Region in Slovakia.