LABORATORY OF FLOW CYTOMETRY, CELL PHENOTYPING AND ENGINEERING

About the lab

Research area of our lab focuses on development and preparation of unique in vitro models (3D and 2D cancer cell lines), derived from human tumor tissues. Our scientists have had several yearexperience with such models from several types of cancer (pancreatic ductal adenocarcinoma, colorectal carcinoma and neuroendocrine tumors). De novo established cancer cell lines are routinely being characterized on in vitro level (by flow cytometry, fluorescence microscopy, protein chip technology, Western blot and PCR analysis) and in vivo by xenotransplantation (grafting) of human cancer cells into athymic (nude, Crl:NU(NCr)-Foxn1nu) mice models, in order to find out histological similarity between xenografts and original tumor tissue. In collaboration with the Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, we are currently working on a project “XenoPig” which will enable to make such models of cancer diseases which are physiologically closer to human being.

These technologies follow the first successful isolation of 3D cancer line (3DiNET) from neuroendocrine pancreas cancer. Independent testing of this cell line, performed by a researcher of our lab is currently in progress in laboratories of Harvard Medical School in Boston, USA. With the development of unique in vitro and in vivo models, we would like to contribute to the global fight against cancer.

Our laboratory was one of the first in the Slovak Republic performing induced pluripotent stem cells technology (iPSc). Our 12-year experience and knowledge is being used in the APVV project and international collaboration projects for development of in vitro models for the research of amyotrophic lateral sclerosis (ALS) and other neurodegenerative diseases. Using this technology, it is possible to prepare infinite number of difficult-to-get cell types needed for disease research – in the case of ALS, these include motor neurons, that are almost impossible to obtain from ALS patients due to high invasiveness.

In our lab, we have recently prepared and fully described the first Slovak iPSc cell line that was registered in human pluripotent stem cells (hPSC) Charité Universitatsmedizin Berlin registry (https://hpscreg.eu/cell-line/ORIONi001-A).

In addition to research activities, all researchers joined the efforts to fight against COVID-19 pandemic.

Staff

Video tour

Watch a video of our laboratory of Flow Cytometry, Cell Phenotyping and Engineering.

Laboratory infrastructure

The lab is equipped with several sophisticated instruments, such as a flow cytometer/sorter FACS Aria II, fluorescence microscope Olympus iX72, light microscope, BSL-2 laminar hoods, cell incubators, PCR thermocyclers and electrophoretic equipment for Western blot and PCR.

The lab offers introduction and training in flow cytometry, magnetic sorting method (MACS), imunohistochemistry, imunocytochemistry, antibody array protein chips, Western blot analysis of protein expression. We also provide training and consultations about derivation of primary cancer cell lines from cancer tissue or stem cells from skin biopsies. As a part of scientific cooperation, we also offer training about in vivo xenotransplantation methods and patent consulting services in the field of biotechnologies.

Virtual tour

Take a virtual tour of our modern Laboratory of Flow Cytometry, Cell Phenotyping and Engineering.

Projects

APVV-17-0037, project title: Development of new in vitro models for amyotrophic lateral sclerosis and testing the safety of neural precursors derived from human induced pluripotent stem cells.

VEGA 1/0279/18, project title: The use of newly developed 3D in vitro cancer cell models in testing of DNA repair inhibiting nanoparticles.  

The most important publications

Strnadel J, Choi S, Fujimura K, Wang H, Zhang W, Wyse M, Wright T, Gross E, Peinado C, Park H.W, Bui J, Kelber J, Bouvet M, Guan K.L, Klemke R.L (2017). eIF5A-PEAK1 signaling regulates YAP1/TAZ protein expression and pancreatic cancer cell growth. Cancer Res 77, 1997–2007. (IF=9,122)

Strnadel J, Carromeu C, Bardy C, Navarro M, Platoshyn A, Glud A.N, Marsala S, Kafka J, Miyanohara A, Kato T, Tadokoro T, Hefferan M.P, Kamizato K, Yoshizumi T, Juhas S, Juhasova J, Ho C.S, Kheradmand T, Chen P, Bohaciakova D, Hruska-Plochan M, Todd A.J, Driscoll S.P, Glenn T.D, Pfaff S.L, Klima J, Ciacci J, Curtis E, Gage F.H, Bui J, Yamada K, Muotri A.R, Marsala M. (2018). Survival of syngeneic and allogeneic iPSC-derived neural precursors after spinal grafting in minipigs. Sci Transl Med 10, 1-14. (IF=16,796)

Strnadel J, Woo S. M, Choi S, Wang H, Grendar M, Fujimura K. (2018). 3D Culture Protocol for Testing Gene Knockdown Efficiency and Cell Line Derivation. Bio-protocol 8, e2874.

Strnadel J, Wang H, Carromeu C, Miyanohara A, Fujimura K, Blahovcova E, Nosal V, Skovierova H, Klemke R,  Halasova E. (2018). Transplantation of Human-Induced Pluripotent Stem Cell-Derived Neural Precursors into Early-Stage Zebrafish Embryos. Journal of molecular neuroscience 65(3), 351–358 (IF=2,678)

Strnadel J, Zahumenska R, Nosal V,  Smolar M, Marcinek J, Kalman M, Juhas S, Juhasova J, Studenovska H, Dumortier H, et al. (2020). Generation of ORIONi001-A induced pluripotent stem cell line for in vitro modeling of sporadic form of amyotrophic lateral sclerosis. Stem. Cell Res. 4, 101981 (IF=4,489)

Zahumenska R, Nosal V, Smolar M, Okajcekova T, Skovierova H, Strnadel J,  Halasova E. (2020). Induced Pluripotency: A Powerful Tool for In Vitro Modeling. International journal of molecular sciences, 21(23), 8910 (IF=4,456)

Kertys M, Grendar M, Horak V, Zidekova N, Kupcova Skalnikova H, Mokry J, Halasova E, Strnadel J. (2021). Metabolomic characterisation of progression and spontaneous regression of melanoma in the melanoma-bearing Libechov minipig model. Melanoma research, 31(2), 140–151 (IF=2,750)