Video tour
Watch a video tour of our Laboratory of Cell Cultures and Regenerative Medicine.
Regenerative medicine is an interdisciplinary field that applies engineering and life science knowledge to promote regeneration of injured tissues. The field of regenerative medicine embraces various strategies, including the use of materials and de novo generated cells to take the place of missing tissue and to contribute for tissue healing. Dental stem cells are great candidate for applications in regenerative medicine because they are relatively easy to access, have a very good ability to proliferate and can differentiate into several tissue types.
In our laboratory, we investigate the biological properties and differentiation potential of human dental stem cells obtained from deciduous and adult teeth. These cells have become as part of our “Biobank for Adult Stem Cell.” We have successfully established methods for isolation, in vitro cultivation, phenotypic characterization, analysis of biological, biochemical and molecular properties of dental stem cells. We use modern methods to analyze complex microRNA and protein profiles that are specific to dental stem cell differentiation processes. We generate new cell lines from dental stem cells cultured in 3D conditions using polymers for future research and commercial purposes. Our priority is the implementation of results obtained from basic medical research into clinical practice. We are the first workplace in Slovakia that specializes on the dental stem cells research and their long-term storage.
Also, we analyze the newly synthetized antitumor agents and study the effect of physical effectors (radiation, plasma) on various types of human cells, as primary cultures as commercially available cell lines (healthy and tumor).
We offer the possibility for practical training: the isolation of DNA, RNA, and microRNA from various types of biological material, protein analysis, using Western blot or protein chips analyses, and immunocytochemical staining. We consult in the field of design experiments for omics analysis of microRNAs and proteins as well as for genotyping DNA/RNA variants by PCR using various detection methods.
We can specify de novo generated cell lines and characterize them at the biological and cytological level by using our modern and high-quality equipment. The following devices are: BSL-2 flow boxes, CO2 incubators, light microscope, deep freezing box and cell counter, which are part of general equipment of our cell culture laboratory. For molecular analyzes, we use PCR and qPCR thermocyclers, agarose/SDS-PAGE apparatus and Western blot transfers, Elisa plate reader, high content imagine system WiscanÒ Hermes and microdissection device – mmiCellCut Plus.
Zahumenska R, Badurova B, Pavelek M, Sojka P, Pavlisova T, Spanik P, Sivonova MK, Novakova S, Strnadel J, Halasova E, Frivaldsky M, Skovierova H. Comparison of pulsed and continuous electromagnetic field generated by WPT system on human dermal and neural cells. Sci Rep. 2024 Mar 6;14(1):5514. doi: 10.1038/s41598-024-56051-z.
Nováková S, Baranovičová E, Hatoková Z, Beke G, Pálešová J, Záhumenská R, Baďurová B, Janíčková M, Strnádel J, Halašová E, Škovierová H. Comparison of Various Extraction Approaches for Optimized Preparation of Intracellular Metabolites from Human Mesenchymal Stem Cells and Fibroblasts for NMR-Based Study. Metabolites. 2024 May 7;14(5):268. doi: 10.3390/metabo14050268.
Novakova S, Czippelova B, Baranovicova E, Sarlinova M, Urbanova A, Hatokova Z, Dzian A, Banovcin P, Strnadel J, Novak P, Horvath G, Halasova E, Skovierova H. Towards optimizing the protocol for untargeted profiling of urine volatiles via gas chromatography-ion mobility spectrometry. A pilot study. J Physiol Pharmacol. 2024 Apr;75(2):215-222. doi: 10.26402/jpp.2024.2.1
Nováková S, Danchenko M, Okajčeková T, Baranovičová E, Kováč A, Grendár M, Beke G, Pálešová J, Strnádel J, Janíčková M, Halašová E, Škovierová H. Comparative Proteomic and Metabolomic Analysis of Human Osteoblasts, Differentiated from Dental Pulp Stem Cells, Hinted Crucial Signaling Pathways Promoting Osteogenesis. Int J Mol Sci 2021; 22(15):7908. doi: 10.3390/ijms22157908
Škovierová H, Pavelek M, Okajčeková T, Pálešová J, Strnádel J, Špánik P, Halašová E, Frivaldský M. The Biocompatibility of Wireless Power Charging System on Human Neural Cells. App Sci 2021; 21(8):3611. doi.org/10.3390/app11083611
Sarlinova M, Baranovicova E, Skalicanova M, Dzian A, Petras M, Lehotsky J, Kalenska D, Racay P, Matakova T, Halasova E. Metabolomic profiling of blood plasma of patients with lung cancer and malignant tumors with metastasis in the lungs showed similar features and promising statistical discrimination against controls. Neoplasma 2021; 68(4):852-860. doi: 10.4149/neo_2021_210103N3
Okajcekova T, Strnadel J, Pokusa M, Zahumenska R, Janickova M, Halasova E, Skovierova H. A Comparative In Vitro Analysis of the Osteogenic Potential of Human Dental Pulp Stem Cells Using Various Differentiation Conditions. Int J Mol Sci 2020; 21(7):2280. doi: 10.3390/ijms21072280
Nova Z, Skovierova H, Strnadel J, Halasova E, Calkovska A. Short-term vs long-term culture of A549 cells for evaluating the effects of Lipopolysaccharide on oxidative stress, surfactant proteins and cathelicidin LL-37. Int J Mol Sci 2020; 21,1148. doi: 10.3390/ijms21031148
Braný D, Dvorská D, Halašová E, Škovierová H. Cold Atmospheric Plasma: A Powerful Tool for Modern Medicine. Int J Mol Sci. 2020;21(8):2932. doi: 10.3390/ijms21082932
Nováková S, Šubr Z, Kováč A, Fialová I, Beke G, Danchenko M. Cucumber mosaic virus resistance: Comparative proteomics of contrasting Cucumis sativus cultivars after long-term infection. J Proteom 2020; 214:103626. doi: 10.1016/j.jprot.2019.103626
Škovierová H, Okajčeková T, Strnádel J, Vidomanová E, Halašová E. Molecular regulation of epithelial- to-mesenchymal transition in tumorigenesis. Int J Mol Med 2018; 41(3):1187-1200. doi: 10.4149/neo_2018_180430N281
Sarlinova M, Halasa M, Mistuna M, Musak L, Iliev R, Slaby O, Mazuchova J, Valentova V, Plank L, Halasova E. miR-21, miR-221 and miR-150 are deregulated in peripheral blood of patients with colorectal cancer. Anticancer Res 2016; 36(10):5449-5454. doi: 10.21873/anticanres.11124
The result of the EU-funded BioMed Martin project is a revolutionary research center that develops new diagnostic and therapeutic methods and at the same time strengthens cooperation between the city of Martin and the Žilina Region in Slovakia.