Watch a video tour of our Laboratory of Pharmacokinetics.
Laboratory of pharmacokinetics deals with two main fields of scientific research activities. It primary focuses on development of analytical methods and their application to preclinical, or clinical research. The laboratory is equipped with triple quadrupole mass spectrometers which are connected to liquid, or gas chromatograph. Such devices are able to analyse compounds at low concentration levels (ng – pg/mL samples) as well as compounds having a broad spectrum of polarity. Our research mainly focuses on pharmacokinetic and toxicological analyses of drugs and their metabolites in biological samples such as blood, plasma or urine. Analytical methods are approved according to the valid regulations (Guideline on bioanalytical method validation – EMA, Bioanalytical Method Validation – FDA) and subsequently applied in various studies and projects.
Another scientific research activity is targeted at metabolomic analyses of biological samples. Our laboratory has implemented methods via commercially available kits from Biocrates Life Science AG (Austria) company used for metabolomic studies of various biological materials, such as blood, plasma, urine, CSF, cell cultures, or tissues. Using metabolomic analyses, we are able to quantify analytes (metabolites) covering a wide range of biologically important compounds, such as amino acids, biogenic amines, acylcarnitines, glycerophospholipids, sphingomyelins, bile acids (primary and secondary, or conjugated and unconjugated) and steroid hormones – glucorticoids, mineralocorticoids, androgens, estrogens and progesterones.
In addition, we provide assistance and consultation with the initial design of pharmacokinetic and toxicological analyses and the design of metabolomic studies. In cooperation with the Laboratory of Bioinformatics and Biostatistics, we participate in the processing and evaluating the metabolomic data and biological interpretation of the results.
The laboratory also provides services and cooperation on a commercial basis. These mainly include targeted metabolomic analyses using kits from Biocrates company. It is also possible to do quantitative analysis of drugs or other exogenous and endogenous analytes in biological samples (blood, plasma, urine, tissues, cell cultures and others). The main advantage is the possibility to optimize analytical methods upon request (detection limit, sample amount, simultaneous analyses of multiple analytes, etc.). In case of interest or further questions, you can contact us by e-mail: firstname.lastname@example.org
Watch a video tour of our Laboratory of Pharmacokinetics.
Laboratory of Pharmacokinetics is equipped with triple quadrupole mass spectrometer with liquid chromatograph (Xevo TQ-S s Acquity 2D UHPLC, Waters) and with triple quadrupole mass spectrometer with gas chromatograph (Scion TQ a 456 GC, Bruker)
In addition, the laboratory is equipped with other essential devices and instruments, such as an instrument for SPE sample pre-treatment (Positive pressure-96 processor, Waters), vacuum sample concentrator (Concentrator plus, Eppendorf), nitrogen sample concentrator (Minivap Gemini, Porvair) and many others.
Take a virtual tour of our modern Laboratory of Pharmacokinetics.
Kertys M, Krivosova M, Ondrejka I, Hrtanek I, Tonhajzerova I, Mokry J. Simultaneous determination offluoxetine, venlafaxine, vortioxetine and their active metabolites in human plasma by LC-MS/MS using one-step sample preparation procedure. Journal of Pharamceutical and Biomedical Analysis, Volume 181, 2020, Article number 113098. DOI: 10.1016/j.jpba.2020.113098.
Kertys M, Urbanova A, Mokry J. Determination of phosphodiesterase inhibitors tadalafil, roflumilast and roflumilast N-Oxide using LC-MS in guinea pig plasma. Journal of Chromatographic Science, Volume 56, Issue 10, 2018, pages 948-954. DOI: 10.1093/chromsci/bmy072.
Zidekova N, Nemcek A, Sutovska M, Mokry J, Kertys M. Development of sensitive and high throughput liquid chromatography-tandem mass spectrometry method for quantification of haloperidol in human plasma with phospholipid removal pretreatment. Journal of Analytical Toxicology, 2020, bkaa124 DOI: 10.1093/jat/bkaa124.
Kertys M, Kosutova P, Mokra D, Mokry J. Development of rapid and high-throughput LC-MS/MS method for quantification of olprinone in rabbit plasma. Biomedical Chromatography, Volume 33, Issue 10, 2019, Article number e4620. DOI: 10.1002/bmc.4620.
Kertys M, Urbanova A, Mestanik M, Tonhajzerova I, Mokry J. Simultaneous determination of total cortisol and cortisone in human plasma by liquid chromatography-tandem mass spectrometry: method development, validation and preliminary clinical application. Current Pharmaceutical Analysis, Volume 15, Issue 4, 2019, Pages 363-370DOI: 10.2174/1573412914666180427094811.
Galba J, Piestansky J, Kovac A, Olesova D, Cehlar O, Kertys M, Kozlik P, Chalova P, Tircova B, Sliz K, Mikus P. Fast and sensitive screening of oxandrolone and its major metabolite 17-epi-oxandrolone in human urine by UHPLC-MS/MS with on-line SPE sample pretreatment. Molecules, Volume 26, Issue 2, 2021, Article number 480. DOI: 10.3390/molecules26020480.
Kertys M, Grendar M, Kosutova P, Mokra D, Mokry J. Plasma based targeted metabolomic analysis reveals alterations of phosphatidylcholines and oxidative stress markers in guinea pig model of allergic asthma. Biochimica et Biophysica Acta-Molecular Basis of Disease, Volume 1866, Issue 1, 2020, Article number 165572. DOI: 10.1016/j.bbadis.2019.165572.
Kertys M, Grendar M, Horak V, Zidekova N, Kupcova Skalnikova H, Mokry J, Halasova E, Strnadel J. Metabolomic characterisation of progression and spontaneous regression of melanoma in the melanoma-bearing Libechov minipig model. Melanoma Research, Volume 31, Issue 2, 2021, Pages 140-151. doi: 10.1097/CMR.0000000000000722.
Mokry J, Urbanova A, Medvedova I, Kertys M, Mikolka P, Kosutova P, Mokra D. Effects of tadalafil (PDE5 inhibitor) and roflumilast (PDE4 inhibitor) on airway reactivity and markers of inflammation in ovalbumin-induced airway hyperresponsiveness in guinea pigs. Journal of Physiology and Pharmacology, Volume 68, Issue 5, 2017, Pages 721-730.
Mokry J, Urbanova A, Kertys M, Mokra D. Inhibitors of phosphodiesterases in the treatment of cough. Respiratory Physiology & Neurobiology, Volume 257, 2018, Pages 107-114. DOI: 10.1016/j.resp.2018.01.008.