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The Laboratory of Metabolomics is a unique workplace that enables monitoring of metabolic processes and characterization of substances in one place, in combination with non-invasive imaging.
Cell metabolism is an essential feature of life and results in the formation of metabolites in a living organism. Analysis of their concentration changes is one of the basic diagnostic tool of modern medicine. Laboratory of Metabolomics offers targeted and non-targeted metabolomics analysis using highly specialized instruments, including a mass spectrometer (with an accuracy of 0.0001 Da) and a 600 MHz NMR spectrometer. We focus mainly on metabolic analyses of low molecular weight substances in tissues, cell cultures and biological fluids (blood plasma, urine, cerebrospinal fluid, tissue extracts, cell media and lysates).
The lab is also equipped with an experimental 7 Tesla scanner based on magnetic resonance enabling non-invasive imaging (MRI) of biological systems but also other types of samples. Imaging sequences allow the monitoring of a wide range of in vivo, ex vivo and in vitro processes and are also suitable for functionalized therapy monitoring using magnetic nanoparticles (theranostics). In addition, using spectroscopic methods (MRS), we can monitor biochemical profiles of the examined tissues, including dynamic pharmacological and metabolic processes in vivo.
Watch a video tour of our Laboratory of Metabolomics.
Bruker Avance III 600 MHz NMR spectrometer
MRI / MRS scanner Bruker BioSpec 70/20 USR 7 T
RWD anaesthesia with isoflurane inhalant
Bruker Q-TOF high-resolution mass spectrometer
Take a virtual tour of our modern Metabolomics Laboratory.
Projects
The most important publications
Antal I, Strbak O, Khmara I, Koneracka M, Kubovcikova M, Zavisova V, Kmetova M, Baranovicova E, and Dobrota D. MRI relaxivity changes of the magnetic nanoparticles induced by different amino acid coatings. Nanomaterials2020, 10:394. doi:10.3390/nano10020394
https://www.mdpi.com/2079-4991/10/2/394
Baranovicova E, Kalenska D, Tomascova A, Holubcikova S, Lehotsky J. Time-related metabolomics study in the rat plasma after global cerebral ischemia and reperfusion: Effect of ischemic preconditioning. IUBMB Life 2020, 72:2010-2023. doi: 10.1002/iub.2340
https://iubmb.onlinelibrary.wiley.com/doi/epdf/10.1002/iub.2340
Hnilicova, P., Strbak, O., Kolisek, M., Kurca, E., Zelenak, K., Sivak, S., Kantorova, E. Current methods of magnetic resonance for noninvasive assessment of molecular aspects of pathoetiology in Muliple Sclerosis. Int J Mol Sci2020, 21:6117. doi: 10.3390/ijms21176117
https://www.mdpi.com/1422-0067/21/17/6117
Kovalska M, Hnilicova P, Kalenska D, Tomascova A, Adamkov M, Lehotsky J: Effect of Methionine Diet on Time-Related Metabolic and Histopathological Changes of Rat Hippocampus in the Model of Global Brain Ischemia. Biomolecules 2020, 10:1-20. doi: 10.3390/biom10081128
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7465067/
Strbak O, Antal I, Khmara I, Koneracka M, Kubovcikova M, Zavisova V, Molcan M, Jurikova A, Hnilicova P, Gombos J, Kadasova N, Dobrota D. Influence of dextran molecular weight on the physical properties of magnetic nanoparticles for hyperthermia and MRI applications. Nanomaterials 2020, 10:2468. doi:10.3390/nano10122468
https://www.mdpi.com/2079-4991/10/12/2468
Strbak O, Balejcikova L, Kmetova M, Gombos J, Trancikova A, Pokusa M, Kopcansky P. Quantification of iron release from native ferritin and magnetoferritin induced by vitamins B2 and C. Int J Mol Sci 2020, 21:6332. doi:10.3390/ijms21176332
https://www.mdpi.com/1422-0067/21/17/6332
Baranovicova E, Galanda T, Galanda M, Hatok J, Kolarovszki B, Richterova R, Racay P. Metabolomic profiling of blood plasma in patients with primary brain tumours: Basal plasma metabolites correlated with tumour grade and plasma biomarker analysis predicts feasibility of the successful statistical discrimination from healthy subjects – a preliminary study. IUBMB Life 2019, 71:1994-2002. doi: 10.1002/iub.2149
https://iubmb.onlinelibrary.wiley.com/doi/full/10.1002/iub.2149
Hnilicova P, Kantorova E, Polacek H, Grendar M, Bittsansky M, Cierny D, Sivak S, Zelenak K, Lehotsky J, Dobrota D, Kurca E. Altered hypothalamic metabolism in early multiple sclerosis – MS spectroscopy study. J Neurol Sci2019, 407:1-9. doi: 10.1016/j.jns.2019.116458
https://pubmed.ncbi.nlm.nih.gov/31622679
Baranovicova E, Kalenska D, Tomascova A, Lehotsky J. Metabolomic study of altered energy metabolism during global forebrain ischemia and ischemic precoditioning in blood plasma in homocysteine treated rats. J Physiol Pharmacol 2018, 69:901-909. doi: 10.26402/jpp.2018.6.04
http://www.jpp.krakow.pl/journal/archive/12_18/pdf/10.26402/jpp.2018.6.04.pdf
Hnilicova P, Povazan M, Strasser B, Andronesi OC, Gajdosik M, Dydak U, Ukropec J, Dobrota D, Trattnig S, Bogner W: Spatial variability and reproducibility of GABA-edited MEGA-LASER 3D- MRSI in the brain at 3T. NMR in Biomedicine 2016, 29(11):1656-1665. doi: 10.1002/nbm.3613
The result of the EU-funded BioMed Martin project is a revolutionary research center that develops new diagnostic and therapeutic methods and at the same time strengthens cooperation between the city of Martin and the Žilina Region in Slovakia.