Video tour
Watch a vide tour of our Laboratory of Proteomics and Mitochondriopathies.
One of the priority research area of our laboratory is proteinological and proteomic analysis of various Parkinson’s disease model systems and biological materials obtained from patients with Parkinson’s disease, Miyoshi syndrome/myopathy, or MIDD (maternaly inherited diabetes and deafness) – MELAS (Mitochondrial Encephalopathy, Lactic Acidosis, Stroke-like episodes) spectrum syndrome.
Due to the fact that these are disorders associated with systemic, metabolic and/or nerve degeneration, which is directly related to damaged mitochondrial homeostasis and cell energy profile, another priority area of research in our laboratory is focused on the etiopathogenesis of primary and secondary mitochondriopathies.
Watch a vide tour of our Laboratory of Proteomics and Mitochondriopathies.
The core technologies used in our laboratory are common techniques of molecular, biological and genetic analyses, which are combine with state-of-the-art MALDI-TOF mass spectrometry, ESI-IT mass spectrometry and NMR spectroscopy. These technologies allow us to analyze both the proteome and the phospho-proteome of cells, which combines very well with the subsequent analysis aimed at evaluating the activity of the signalome using a chip technology.
Measurements of oxygen consumption at the cellular level (basal respiration, maximum respiration capacity, respiratory reserve) and bioenergetic profile are usually determined in cell cultures or in white blood cells isolated from venous blood using an Oroboros O2k respirometer. Circulating cells in patients are specific in a way that come into contact with all organs in the body, and thus usually react to various pathological conditions and cause changes. The outputs of this method can serve as biomarker (s) of complex cellular energy.
Mitochondrial dysfunction (so called mitochondrial respiratory disorders) is associated with various degenerative diseases (such as mitochondrial encephalopathy, Parkinson’s and Alzheimer’s disease, Kearns-Sayre syndrome, MIDD / MELAS syndrome, diabetes, muscular dystrophies, ALS, schizophrenia). The study of this dysfunction in relation to the above-mentioned diseases is of great importance, both from a scientific or clinical point of view. Determination of bioenergetic profile is also justified in the field of preventive medicine, for example, in diagnostics, therapy and research of metabolic syndrome and obesity, or cardiovascular diseases.
Take a virtual tour of our modern Laboratory of Proteomics and Mitochondriopathies.
Government of the Slovak Republic – project “Back home”: Influence of deletion of Na + / Mg2 + exchanger SLC41A1 and mitochondrial Na + -dependent Mg2 + exporter SLC41A3 on brain morphology and molecular composition in mouse MPTP-induced Parkinsonian model; solution period: 2016-2017.
(Principal inestigator: doc. RNDr. Martin Kolísek, Dr.rer.nat.(Genet.))
APVV-23-0368: Mitochondrial transplantation, an innovative therapeutical strategy of Alzheimer’s disease.
(Principal inestigator: doc. RNDr. Martin Kolísek, Dr.rer.nat.(Genet.))
APVV-19-0222: Determination of mitochondrial fitness in the diagnostics and prediction of Parkinson’s disease.
(Principal inestigator: doc. RNDr. Martin Kolísek, Dr.rer.nat.(Genet.))
APVV-SK-AT-20-0010: Multiparametric examinations of Miyoshi myopathy using magnetic resonance modalities.
(Principal inestigator: Ing. Petra Hnilicová, PhD.)
VEGA 1/0039/23: Identification of components of magnesium homeostasis and study of their regulation in blood-brain barrier cells.
(Principal inestigator: MUDr. Michal Cibulka, PhD.)
VEGA 1/0554/19: The role of the STAT3 signalling pathway in the regulation of the Na + / Mg2 + promoter of the SLC41A1 exchanger: from inflammation to Parkinson’s disease.
(Principal inestigator: doc. RNDr. Martin Kolísek, Dr.rer.nat.(Genet.))
CO-INVESTIGATOR PROJECTS:
APVV-SK-KR-18-0001: Mitochondrial-like nanoparticles in the treatment of Parkinson’s disease and other degenerative diseases.
(Principal inestigator: doc. Ing. Zuzana Tatarková, PhD.)
Brodnanova M, Cibulka M, Grendar M, Gondas E, Kolisek M. IL-6 Does Not Influence the Expression of SLC41A1 and Other Mg-Homeostatic Factors. Int J Mol Sci. 2024 Dec 11;25(24):13274. doi: 10.3390/ijms252413274. PMID: 39769039; PMCID: PMC11675721.
Hnilicova P, Grendar M, Turcanova Koprusakova M, Trancikova Kralova A, Harsanyiova J, Krssak M, Just I, Misovicova N, Hikkelova M, Grossmann J, Spalek P, Meciarova I, Kurca E, Zilka N, Zelenak K, Bogner W, Kolisek M. Brain of miyoshi myopathy/dysferlinopathy patients presents with structural and metabolic anomalies. Sci Rep. 2024 Aug 20;14(1):19267. doi: 10.1038/s41598-024-69966-4. PMID: 39164335; PMCID: PMC11336102.
Cibulka M, Brodnanova M, Halasova E, Kurca E, Kolisek M, Grofik M. The Role of Magnesium in Parkinson’s Disease: Status Quo and Implications for Future Research. Int J Mol Sci. 2024 Aug 1;25(15):8425. doi: 10.3390/ijms25158425. PMID: 39125993; PMCID: PMC11312984.
Parvanovova P, Hnilicova P, Kolisek M, Tatarkova Z, Halasova E, Kurca E, Holubcikova S, Koprusakova MT, Baranovicova E. Disturbances in Muscle Energy Metabolism in Patients with Amyotrophic Lateral Sclerosis. Metabolites. 2024 Jun 23;14(7):356. doi: 10.3390/metabo14070356. PMID: 39057679; PMCID: PMC11278632.10.3390/ijms24043325. PMID: 36834741; PMCID: PMC9958721.
Kubáňová L, Bielik V, Hric I, Ugrayová S, Šoltys K, Rádiková Ž, Baranovičová E, Grendár M, Kolisek M, Penesová A. Gut Microbiota and Serum Metabolites in Individuals with Class III Obesity Without Type 2 Diabetes Mellitus: Pilot Analysis. Metab Syndr Relat Disord. 2023 Jun;21(5):243-253. doi: 10.1089/met.2022.0071. Epub 2023 Apr 20. PMID: 37083403.
Hnilicova P, Kantorova E, Sutovsky S, Grofik M, Zelenak K, Kurca E, Zilka N, Parvanovova P, Kolisek M. Imaging Methods Applicable in the Diagnostics of Alzheimer’s Disease, Considering the Involvement of Insulin Resistance. Int J Mol Sci. 2023 Feb 7;24(4):3325. doi:
Grofik M, Cibulka M, Oleksakova J, Sivak S, Cierny D, Tatarkova Z, Grendar M, Nosal V, Ruzinak R, Kurca E, Kolisek M. Oxidative stress parameters and their relation to motor subtype of Parkinson’s disease and levodopa treatment status. Gen Physiol Biophys. 2023 Jan;42(1):77-85. doi: 10.4149/gpb_2022051. PMID: 36705307.
Touyz RM, Wolf F, Maier JA, Rosanoff A, West C, Elin RJ, Micke O, Baniasadi S, Barbagallo M, Campbell E, Cheng FC, Costello RB, Gamboa-Gomez C, Guerrero-Romero F, Gletsu-Miller N, von Ehrlich B, Iotti S, Kahe K, Kim DJ, Kisters K, Kolisek M, Kraus A, Maj-Zurawska M, Merolle L, Nechifor M, Pourdowlat G, Shechter M, Song Y, Teoh YP, Wallace TC, Yokota K; MaGNet Global Magnesium Project (MaGNet). Reply to “Recommendation on an updated standardization of serum magnesium reference ranges,” Jeroen H.F. de Baaij et al. Eur J Nutr. 2022 Dec;61(8):4235-4237. doi: 10.1007/s00394-022-03005-8. Epub 2022 Oct 7. PMID: 36207423.
Ilenwabor BP, Franken GAC, Sponder G, Bos C, Racay P, Kolisek M, Hoenderop JGJ, de Baaij JHF. SLC41A1 knockout mice display normal magnesium homeostasis. Am J Physiol Renal Physiol. 2022 Nov 1;323(5):F553-F563. doi: 10.1152/ajprenal.00101.2022. Epub 2022 Sep 1. PMID: 36049064.
Rosanoff A, West C, Elin RJ, Micke O, Baniasadi S, Barbagallo M, Campbell E, Cheng FC, Costello RB, Gamboa-Gomez C, Guerrero-Romero F, Gletsu-Miller N, von Ehrlich B, Iotti S, Kahe K, Kim DJ, Kisters K, Kolisek M, Kraus A, Maier JA, Maj-Zurawska M, Merolle L, Nechifor M, Pourdowlat G, Shechter M, Song Y, Teoh YP, Touyz RM, Wallace TC, Yokota K, Wolf F; MaGNet Global Magnesium Project (MaGNet). Recommendation on an updated standardization of serum magnesium reference ranges. Eur J Nutr. 2022 Oct;61(7):3697-3706. doi: 10.1007/s00394-022-02916-w. Epub 2022 Jun 10. PMID: 35689124; PMCID: PMC9186275.
Ugrayová S, Švec P, Hric I, Šardzíková S, Kubáňová L, Penesová A, Adamčáková J, Pačesová P, Horáková J, Kolenová A, Šoltys K, Kolisek M, Bielik V. Gut Microbiome Suffers from Hematopoietic Stem Cell Transplantation in Childhood and Its Characteristics Are Positively Associated with Intra-Hospital Physical Exercise. Biology (Basel). 2022 May 21;11(5):785. doi: 10.3390/biology11050785. PMID: 35625513; PMCID: PMC9138603.
Bielik V, Hric I, Ugrayová S, Kubáňová L, Putala M, Grznár Ľ, Penesová A, Havranová A, Šardzíková S, Grendar M, Baranovičová E, Šoltys K, Kolisek M. Effect of High-intensity Training and Probiotics on Gut Microbiota Diversity in Competitive Swimmers: Randomized Controlled Trial. Sports Med Open. 2022 May 10;8(1):64. doi: 10.1186/s40798-022-00453-8. PMID: 35536489; PMCID: PMC9091066.
Cibulka M, Brodnanova M, Grendar M, Necpal J, Benetin J, Han V, Kurca E, Nosal V, Skorvanek M, Vesely B, Stanclova A, Lasabova Z, Pös Z, Szemes T, Stuchlik S, Grofik M, Kolisek M. Alzheimer’s Disease-Associated SNP rs708727 in SLC41A1 May Increase Risk for Parkinson’s Disease: Report from Enlarged Slovak Study. Int J Mol Sci. 2022 Jan 29;23(3):1604. doi: 10.3390/ijms23031604. PMID: 35163527; PMCID: PMC8835868.
Jurecek L, Rajcigelova T, Kozarova A, Werner T, Vormann J, Kolisek M. Beneficial effects of an alkaline topical treatment in patients with mild atopic dermatitis. J Cosmet Dermatol. 2021 Sep;20(9):2824-2831. doi: 10.1111/jocd.13936. Epub 2021 Jan 14. PMID: 33448109.
Turčanová Koprušáková M, Grofik M, Kantorová E, Jungová P, Chandoga J, Kolisek M, Valkovič P, Škorvánek M, Ploski R, Kurča E, Sivák Š. Atypical presentation of Charcot-Marie-Tooth disease type 1C with a new mutation: a case report. BMC Neurol. 2021 Jul 27;21(1):293. doi: 10.1186/s12883-021-02316-3. PMID: 34311727; PMCID: PMC8314550.
Bielik V, Grendar M, Kolisek M. A Possible Preventive Role of Physically Active Lifestyle during the SARS-CoV-2 Pandemic; Might Regular Cold-Water Swimming and Exercise Reduce the Symptom Severity of COVID-19? Int J Environ Res Public Health. 2021 Jul 4;18(13):7158. doi: 10.3390/ijerph18137158. PMID: 34281096; PMCID: PMC8297290.
Bielik V, Kolisek M. Bioaccessibility and Bioavailability of Minerals in Relation to a Healthy Gut Microbiome. Int J Mol Sci. 2021 Jun 24;22(13):6803. doi: 10.3390/ijms22136803. PMID: 34202712; PMCID: PMC8268569.
Šoltys K, Lendvorský L, Hric I, Baranovičová E, Penesová A, Mikula I, Bohmer M, Budiš J, Vávrová S, Grones J, Grendar M, Kolísek M, Bielik V. Strenuous Physical Training, Physical Fitness, Body Composition and Bacteroides to Prevotella Ratio in the Gut of Elderly Athletes. Front Physiol. 2021 Jun 22;12:670989. doi: 10.3389/fphys.2021.670989. PMID: 34239449; PMCID: PMC8257935.
Becker SK, Sponder G, Sandhu MA, Trappe S, Kolisek M, Aschenbach JR. The Combined Influence of Magnesium and Insulin on Central Metabolic Functions and Expression of Genes Involved in Magnesium Homeostasis of Cultured Bovine Adipocytes. Int J Mol Sci. 2021 May 31;22(11):5897. doi: 10.3390/ijms22115897. PMID: 34072724; PMCID: PMC8199494.
Hric I, Ugrayová S, Penesová A, Rádiková Ž, Kubáňová L, Šardzíková S, Baranovičová E, Klučár Ľ, Beke G, Grendar M, Kolisek M, Šoltys K, Bielik V. The Efficacy of Short-Term Weight Loss Programs and Consumption of Natural Probiotic Bryndza Cheese on Gut Microbiota Composition in Women. Nutrients. 2021 May 21;13(6):1753. doi: 10.3390/nu13061753. PMID: 34064069; PMCID: PMC8224276.
Wolf FI, Maier JA, Rosanoff A, Barbagallo M, Baniasadi S, Castiglioni S, Cheng FC, Day SC, Costello RB, Dominguez LJ, Elin RJ, Gamboa-Gomez C, Guerrero-Romero F, Kahe K, Kisters K, Kolisek M, Kraus A, Iotti S, Mazur A, Mercado-Atri M, Merolle L, Micke O, Gletsu-Miller N, Nielsen F, O-Uchi J, Piazza O, Plesset M, Pourdowlat G, Rios FJ, Rodriguez-Moran M, Scarpati G, Shechter M, Song Y, Spence LA, Touyz RM, Trapani V, Veronese N, von Ehrlich B, Vormann J, Wallace TC, Cmer Center For Magnesium Education Research, Gesellschaft Für Magnesium-Forschung E V Germany, Sdrm Society International Society For The Development Of Research On Magnesium. The magnesium global network (MaGNet) to promote research on magnesium in diseases focusing on covid-19. Magnes Res. 2021 May 1;34(2):90-92. doi: 10.1684/mrh.2021.0479. PMID: 34524085; PMCID: PMC10617598.
Jurek S, Sandhu MA, Trappe S, Bermúdez-Peña MC, Kolisek M, Sponder G, Aschenbach JR. Optimizing adipogenic transdifferentiation of bovine mesenchymal stem cells: a prominent role of ascorbic acid in FABP4 induction. Adipocyte. 2020 Dec;9(1):35-50. doi: 10.1080/21623945.2020.1720480. PMID: 31996081; PMCID: PMC6999845.
Tatarkova Z, de Baaij JHF, Grendar M, Aschenbach JR, Racay P, Bos C, Sponder G, Hoenderop JGJ, Röntgen M, Turcanova Koprusakova M, Kolisek M. Dietary Mg2+ Intake and the Na+/Mg2+ Exchanger SLC41A1 Influence Components of Mitochondrial Energetics in Murine Cardiomyocytes. Int J Mol Sci. 2020 Nov 3;21(21):8221. doi: 10.3390/ijms21218221. PMID: 33153064; PMCID: PMC7663288.
Hnilicová P, Štrbák O, Kolisek M, Kurča E, Zeleňák K, Sivák Š, Kantorová E. Current Methods of Magnetic Resonance for Noninvasive Assessment of Molecular Aspects of Pathoetiology in Multiple Sclerosis. Int J Mol Sci. 2020 Aug 25;21(17):6117. doi: 10.3390/ijms21176117. PMID: 32854318; PMCID: PMC7504207.
The result of the EU-funded BioMed Martin project is a revolutionary research center that develops new diagnostic and therapeutic methods and at the same time strengthens cooperation between the city of Martin and the Žilina Region in Slovakia.