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Watch a video tour of our Laboratory of Pharmacokinetics.
Laboratory of pharmacokinetics deals with two main fields of scientific research activities. It primary focuses on development of analytical methods and their application to preclinical, or clinical research. The laboratory is equipped with triple quadrupole mass spectrometers which are connected to liquid, or gas chromatograph. Such devices are able to analyse compounds at low concentration levels (ng – pg/mL samples) as well as compounds having a broad spectrum of polarity. Our research mainly focuses on pharmacokinetic and toxicological analyses of drugs and their metabolites in biological samples such as blood, plasma or urine. Analytical methods are approved according to the valid regulations (Guideline on bioanalytical method validation – EMA, Bioanalytical Method Validation – FDA) and subsequently applied in various studies and projects.
Another scientific research activity is targeted at metabolomic analyses of biological samples. Our laboratory has implemented methods via commercially available kits from Biocrates Life Science AG (Austria) company used for metabolomic studies of various biological materials, such as blood, plasma, urine, CSF, cell cultures, or tissues. Using metabolomic analyses, we are able to quantify analytes (metabolites) covering a wide range of biologically important compounds, such as amino acids, biogenic amines, acylcarnitines, glycerophospholipids, sphingomyelins, bile acids (primary and secondary, or conjugated and unconjugated) and steroid hormones – glucorticoids, mineralocorticoids, androgens, estrogens and progesterones.
In addition, we provide assistance and consultation with the initial design of pharmacokinetic and toxicological analyses and the design of metabolomic studies. In cooperation with the Laboratory of Bioinformatics and Biostatistics, we participate in the processing and evaluating the metabolomic data and biological interpretation of the results.
The laboratory also provides services and cooperation on a commercial basis. These mainly include targeted metabolomic analyses using kits from Biocrates company. It is also possible to do quantitative analysis of drugs or other exogenous and endogenous analytes in biological samples (blood, plasma, urine, tissues, cell cultures and others). The main advantage is the possibility to optimize analytical methods upon request (detection limit, sample amount, simultaneous analyses of multiple analytes, etc.). In case of interest or further questions, you can contact us by e-mail: martin.kertys@uniba.sk
Watch a video tour of our Laboratory of Pharmacokinetics.
Laboratory of Pharmacokinetics is equipped with triple quadrupole mass spectrometer with liquid chromatograph (Xevo TQ-S s Acquity 2D UHPLC, Waters) and with triple quadrupole mass spectrometer with gas chromatograph (Scion TQ a 456 GC, Bruker)
In addition, the laboratory is equipped with other essential devices and instruments, such as an instrument for SPE sample pre-treatment (Positive pressure-96 processor, Waters), vacuum sample concentrator (Concentrator plus, Eppendorf), nitrogen sample concentrator (Minivap Gemini, Porvair) and many others.
Take a virtual tour of our modern Laboratory of Pharmacokinetics.
R2 project – 09I03-03-V04-00207: Enhancing the management of psychiatric disorders through pharmacogenomics and therapeutic drug monitoring.(Principal Investigator: PharmDr. Michaela Krivošová, PhD.)
VEGA 1/0049/25: Application of innovative approaches in the diagnosis and management of paediatric mycobacteriosis.
(Principal investigator: Mgr. Matúš Dohál, PhD.)
VEGA 1/0029/24: Improving the effectiveness of antithrombotic therapy using a mini-invasive sampling technique.
(Principal investigator: PharmDr. Martin Kertys, PhD.)
VEGA 1/0081/20: Monitoring of efficacy during antiplatelet and anticoagulant therapy.
(Principal investigator: PharmDr. Martin Kertys, PhD.)
CO-INVESTIGATOR PROJECTS:
APVV-18-0084: Resistance to anti-tuberculotics – new possibilities of its detection and therapeutic management.
(Principal investigator: prof. MUDr. Mgr. Juraj Mokrý, PhD.)
VEGA 1/0255/18: Analysis of drug levels and laboratory markers in selected psychiatric disorders.
(Principal investigator: prof. MUDr. Mgr. Juraj Mokrý, PhD.)
Mokrá D, Adamčáková J, Bálentová S, Barošová R, Hanusrichterová J, Žideková N, Mikolka P, Mokrý J, Kertys, M. Novel pilot study on plasma metabolites and biomarkers in a rat model of silica-induced lung inflammation and fibrosis. Biochimica et Biophysica Acta (BBA)-General Subjects 2024, 1868(12), 130729. doi: 10.1016/j.bbagen.2024.130729
Simko P, Leskanicova A, Suvakova-Nunhart M, Koval J, Zidekova N, Karasova M, Majerova P, Verboova L, Blicharova A, Kertys M, Barvik I, Kovac A, Kiskova T. The First In Vivo Study Shows That Gyrophoric Acid Changes Behavior of Healthy Laboratory Rats. International Journal of Molecular Sciences 2024, 25(12), 6782. doi: 0.3390/ijms25126782
Žideková N, Pršo K, Babálová L, Sivák Š, Kurča E, Mokrý J, Nosáľ V, Kertys M. Volumetric Absorptive Microsampling Technique in the LC-MS Determination of Direct Oral Anticoagulants. Acta Medica Martiniana 2023, 23(1), 23-31. doi: 10.2478/acm-2023-0004
Žideková N, Pršo K, Brisudová K, Babálová L, Bolek T, Sivák Š, Kurča E, Mokrý J, Samoš M, Nosáľ V, Kertys M. A high‐throughput liquid chromatography‐tandem mass spectrometry method for simultaneous determination of direct oral anticoagulants in human plasma. Journal of Separation Science 2023, 46(13), 2300084. doi: 10.1002/jssc.202300084
Pršo K, Žideková N, Porvazník I, Solovič I, Mokrý J, Kertys M. A high‐throughput LC–MS/MS method for simultaneous determination of isoniazid, ethambutol and pyrazinamide in human plasma. Rapid Communications in Mass Spectrometry 2023; 37(2), e9425. doi: 10.1002/rcm.9425
Kertys M, Žideková N, Pršo K, Maráková K, Kmeťová K, Mokrý J. Simultaneous determination of caffeine and its metabolites in rat plasma by UHPLC‐MS/MS. Journal of Separation Science 2021; 44(23), 4274-4283. doi: 10.1002/jssc.202100604
Galba J, Piestansky J, Kovac A, Olesova D, Cehlar O, Kertys M, Kozlik P, Chalova P, Tircova B, Sliz K, Mikus P. Fast and sensitive screening of oxandrolone and its major metabolite 17-epi-oxandrolone in human urine by UHPLC-MS/MS with on-line SPE sample pretreatment. Molecules, Volume 26, Issue 2, 2021, Article number 480. DOI: 10.3390/molecules26020480.
Kertys M, Grendar M, Horak V, Zidekova N, Kupcova Skalnikova H, Mokry J, Halasova E, Strnadel J. Metabolomic characterisation of progression and spontaneous regression of melanoma in the melanoma-bearing Libechov minipig model. Melanoma Research, Volume 31, Issue 2, 2021, Pages 140-151. doi: 10.1097/CMR.0000000000000722.
Kertys M, Krivosova M, Ondrejka I, Hrtanek I, Tonhajzerova I, Mokry J. Simultaneous determination offluoxetine, venlafaxine, vortioxetine and their active metabolites in human plasma by LC-MS/MS using one-step sample preparation procedure. Journal of Pharamceutical and Biomedical Analysis, Volume 181, 2020, Article number 113098. DOI: 10.1016/j.jpba.2020.113098.
Zidekova N, Nemcek A, Sutovska M, Mokry J, Kertys M. Development of sensitive and high throughput liquid chromatography-tandem mass spectrometry method for quantification of haloperidol in human plasma with phospholipid removal pretreatment. Journal of Analytical Toxicology, 2020, bkaa124 DOI: 10.1093/jat/bkaa124.
Kertys M, Grendar M, Kosutova P, Mokra D, Mokry J. Plasma based targeted metabolomic analysis reveals alterations of phosphatidylcholines and oxidative stress markers in guinea pig model of allergic asthma. Biochimica et Biophysica Acta-Molecular Basis of Disease, Volume 1866, Issue 1, 2020, Article number 165572. DOI: 10.1016/j.bbadis.2019.165572.
The result of the EU-funded BioMed Martin project is a revolutionary research center that develops new diagnostic and therapeutic methods and at the same time strengthens cooperation between the city of Martin and the Žilina Region in Slovakia.